|Publication of Annual Report for 2014 (June 2015)|
|On 26 May 2015 the Administrative Board approved the 2014 Annual Report on the Foundation's activities as well as the financial statements for 2014. A total of Fr. 401,912.85 was paid out for research projects. Four new projects were approved and eight final project reports were submitted. The Administrative Board and the Evaluation Committee were re-elected for a further 4 year period.|
Annual Report for 2014 | PDF version
|New project (December 2014)|
Validation of a human stem-cell based pluripotency test using a bioreactor-based culturing system instead of a murine model to effect the development of embryoid bodies into teratomasAfter stem cells from donor tissue have been isolated (or cultured) it must be checked whether they have maintained their typical capacity to differentiate into various types of tissue (pluripotency). According to international guidelines, such tests for pluripotency of stem cells are normally carried out using mice with a non-functional immune system.
Prof. Christian de Geyter, University Hospital Basle, Department of Biomedicine, 4031 Basle, Switzerland
The authors propose to develop a new bioreactor-based system which will enable researchers to examine the differentiation of stem cells into various types of tissue in vitro.
|Completion of a project (December 2014)|
Antibody phage selection strategy for application in non-specialized laboratoriesMost antibodies used in research are still produced by immunizing animals. Prof. Heinis and his team succeeded in the development of an antibody scFv phage display library that may be distributed to laboratories free of charge and without any intellectual property (IP) constraints. From this library, antibodies to targets of choice can be isolated in vitro, omitting standard techniques based on animal immunization. In addition, they developed a phage display selection strategy with significantly fewer experimental steps that should facilitate the in vitro generation of affinity ligands by non-experts. The proposed method should replace animal experiments that are commonly performed to develop polyclonal and monoclonal antibodies.
Prof. Dr. Christian Heinis, Laboratory of Therapeutic Peptides and Proteins, EPFL, Lausanne, Switzerland
|Genetic manipulation of the human airway epithelium – a paradigmatic system to study host responses to human respiratory viruses (November 2014)|
3R-Info-Bulletin 53The airway epithelium is the main port of entry for many respiratory pathogens and an important barrier to infection. Experimental systems that are suitable for studying basic virus-host interactions are scarce and are still preferentially performed in animal models. Prof. Thiel and his team succeeded to establish a unique in-vitro airway epithelial-cell culturing system that permits the molecular analysis of host-pathogen interactions at the port of entry of many respiratory pathogens.
3R-Info Bulletin 53 | Project 128-11
|New project (October 2014)|
In vitro alternatives to in vivo bioconcentration testing with fish: restricted to rainbow trout or broadly applicable? Xenobiotic substances are chemical compounds that accumulate as foreign substances (e.g. toxins) in organisms where they are normally not found. According to OECD Guidelines (TG 305, Guidelines for Testing of Chemicals, Degradation and Accumulation), the potential risks of such substances must be tested on animals. A large number of animals are used for such tests.
Prof. Helmut Segner, University of Berne, Centre for Fish and Wildlife Health, 3012 Berne, Switzerland
The project leader has already developed a liver cell culture system using cells from trout, a cold-water fish, whereby the testing can be carried out in vitro (Project 108-07). The researchers now propose to adapt this system for liver cell cultures from carp, a warm-water fish, in order to enable the in vitro testing to be carried out in various other parts of the world.
|New project (October 2014)|
Hydrocephalus simulator for testing active ventriculoperitoneal shunts The present generation of valves and shunts used for treating hydrocephalus is out-of-date. Blockages often occur in the artificial drainage system for transferring excess cerebral fluid from the brain to the abdominal cavity. As a rule, the reliability of new products or systems is tested on larger animals.
Dr. Marianne Schmid Daners, Zurich Federal Institute of Technology, Institute for Dynamic Systems and Control, 8092 Zurich, Switzerland
In order to alleviate the need for such animal testing, a new platform will be developed consisting of a simulator which can be used for testing ventriculoperitoneal shunts in vitro.
|New project (October 2014)|
An in vitro micro-vascular model mimicking the endothelial barrierThe walls of the blood vessels that are lined with endothelial cells, which in turn are covered with pericytes, control the transfer of substances (nutrients, gases such as oxygen, etc.) in both directions (from the blood to the tissue cells and vice versa). Cells also pass through this barrier, e.g. antibody-producing cells, and access the tissue from the blood to eliminate pathogenic organisms that have entered the system. As a rule, studies of the mechanisms whereby substances and cells pass through the barrier involve the use of laboratory animals, mainly transgenic mice.
Dr. Marietta Herrmann, AO Research Institute Davos, 7270 Davos Platz, Switzerland
The aim of this research project is to develop a new in vitro culture system for the blood vessel wall whereby such studies can be carried out in vitro.
|Publication of Annual Report for 2013 (June 2014)|
|On 6 May 2014 the Administrative Board approved the 2013 Annual Report on the Foundation's activities as well as the financial statements for 2013. A total of Fr. 568,479 was paid out for research projects. Four new projects were approved and three final project reports were submitted. Moreover, the Administrative Board decided on a major overhaul of the Administrative Board and the Evaluation Committee.|
Annual Report for 2013 | PDF version
|A new in-vitro approach to the study of brain tumours: an alternative to in-vivo experiments in animals (June 2014)|
3R-Info-Bulletin 52In humans, glioblastomas are the most common and most aggressive type of brain tumours. Animal experimentation as part of research into glioblastomas causes extreme suffering since it involves implanting a tumour in the brain of a mouse. Together with his research team at the University of Geneva, Dr. Olivier Preynat-Seauve has succeeded in developing a cell culture model whereby the interaction between the tumour cells and nerve tissue can be simulated. Through this model, experiments that cause considerable suffering to the mice should become superfluous.
3R-Info Bulletin 52 | Project 115-09
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